THE PROBLEM
Challenges in Recombinant Protein Production
Producing recombinant proteins suitable for research or industrial applications remains technically challenging.
E.coli
Fast and cost-effective, but often leads to misfolded proteins and lacks post-translational modifications.
Insect / CHO systems
Produce functional proteins, but processes are slow, costly, and complex to implement.
Traditional yeast systems
Promising approach, but often unstable and require long optimization cycles.
ProteoY has developed a platform specifically designed to overcome these limitations.
OUR SOLUTION
The ProteoY Platform
🟢 Ligase-free cloning technology
🟢 Stable clones with rapid expression
🟢Self-selected multi-copy plasmid enabling high protein yields
🟢 Eukaryotic expression system (proper folding machinery, no inclusion bodies)
ProteoY is a yeast-based protein expression platform enabling fast, stable, and reproducible production of complex proteins and protein assemblies.
Designed for proteins that conventional systems struggle to produce.
THE PROCESS
How ProteoY Works
1
Ligase-free cloning
Single-step in vivo gene recombination directly into the expression plasmid.
No E. coli step required.
2
Self-selected plasmid maintenance
A proprietary auto-selection system ensures stable plasmid retention in rich media.
3
Expression from a high-copy-number plasmid for maximum yield
Maximized protein expression from self-maintained episomal constructs.
4
Rapid induction & purification
Efficient induction followed by standard purification workflows using customizable tags.
WHY PROTEOY
Key Advantages
7 days from gene (PCR product or synthetic DNA) to protein production system
Functional, properly folded proteins with no size limitation
Compatible with complex, non-standard, and toxic proteins
Multi-protein expression (up to 4 proteins simultaneously)
Stable expression in rich media (high biomass yield)
Reproducible and easily scalable results
USE CASES
Applications
Health & biotechnology
Scientific research
Industrial biotechnology
Foodtech
Energy & environment
Agriculture
Materials & biomaterials
Defense & biosecurity
SCIENTIFIC VALIDATION
Scientifically Validated Technology
The ProteoY expression platform is based on advanced research conducted at the University of Cambridge, combining decades of expertise in yeast biology with innovative plasmid engineering.
A Saccharomyces cerevisiae autoselection system for optimised recombinant protein expression.
Geymonat M, Spanos A and Sedgwick S. Gene. (2007) 399:120-128.
University of Cambridge
Research & Development
Production of mitotic regulators using an autoselection system for protein expression in budding yeast.
Geymonat M, Spanos A and Sedgwick S. Methods Mol Biol. (2009) 545: 63-80
Start your protein production project
Discuss your protein expression needs with our team.