Cloning Workflow
A simplified process from DNA fragment to functional protein.
Stable
strain: the plasmid is maintained by self-selection and the protein induced in
presence of galactose
Key Advantages
No ligase
In vivo recombination eliminates the need for enzymatic ligation, reducing the number of steps and potential failure points.
No restriction enzymes
Sequence-independent cloning, without constraints related to restriction sites.
Faster cloning
Eliminates the need for bacteria to construct/amplify the plasmid, significantly reducing the time between design and obtaining a functional construct.
